Type: HPLC Application #: 108520
Detection: UV, 214nm Conditions: eluent A: aqueous 0.1% TFA, eluent B: 40% acetonitrile, 0.1% TFA, gradient from 20 to 60% B in 30min (1.33% per min), then increase 15% / min for 1min, 0.43% / min for 35min, 10% / min for 1min Authors: Mazrimas JA Source: J Liquid Chromatogr 7 (1984) 907 - 916
Details
Legend: Fig. 1. Separation of HMG proteins, A. HMG 17, B. HMG 14, C. several peptides (fragments of histone or HMG proteins), E. and G. (and possibly I.) variants of HMG 2, H. and J. (perhaps also D. and F.)